Master Mixes for Real Time PCR Applications

Learn about Agilent’s range of master mixes for your real time PCR workflows. Our Brilliant Master Mix kits provide high specificity, speed and reproducibility, while the Brilliant HRM Ultra-Fast Loci Mixes enable you to mix-and-go faster with total confidence, even for the most difficult genotypes.  The Brilliant Master Mix kits come with convenient, pre-blended formulations and an enhanced rapid start capability to save time and reduce primer-dimer. The kits include novel, fast Taq mutant for qPCR results in less than 35 minutes. We also offer the Brilliant Master Mixes with ROX in low and high concentrations of ROX reference dye which help increase sensitivity of detection to low copy numbers.

  1. Ultra-Fast qRT-PCR Kits

  2. Ultrafast qPCR Kits

  3. Multiplex qPCR

  4. High Resolution Melt Analysis

 

cDNA synthesis Kit

AccuScript High-Fidelity Reverse Transcriptase

Discover AccuScript High-Fidelity Reverse Transcriptase (RT). AccuScript delivers 3.7 times more accuracy than other commercially available RTs and 6.6 times more accuracy than AMV RT. The AccuScript PfuUltra II RT-PCR kit provides a complete system in applications that demand high fidelity, specificity, and throughput.

Features

  • MMLV-RT derivative combined with 3’-5’ exonuclease reduces errors

  • High yields of full length cDNA up to 20 Kb

  • RT-PCR kit synthesizes cDNA from 10 to 1000 ng of total RNA or 0.1 to 10 ng of poly(A)+ RNA

  • Stringent quality control procedures ensure high yields of premium cDNA

Specifications

High Fidelity

  • Yes

Long-read

  • 20 Kb

Multiple Temperature Activity

  • No

Reactions per Kit

  • 50-200

Thermal Cycler Type

  • Any

AffinityScript cDNA Synthesis Kit

The AffinityScript qPCR cDNA Synthesis Kit offers efficient conversion of RNA to cDNA. Fully optimized for real-time quantitative PCR (qPCR) applications, the kit utilizes our qPCR-grade AffinityScript Multiple Temperature Reverse Transcriptase. It includes a buffer system optimized for real-time qPCR.

The streamlined protocol produces cDNA in 15 minutes with linear detection from 3 pg to 3 µg of total RNA. The easy-to-use process generates up to 12 kb cDNA in 25 minutes or less. Available as a first-strand synthesis kit and a two-step QRT-PCR master mix format, using SYBR Green or probe-based detection.

Features

  • Optimized for real-time quantitative PCR (qPCR) application

  • Produces cDNA in 15 minutes with linear detection from 3 pg to 3 µg total RNA

  • Creates up to 12 kb cDNA in 25 minutes or less

  • Available as a first-strand synthesis kit and a two-step QRT-PCR master mix format

Specifications

High Yield for GC-rich Sequences

  • Yes

Long-read

  • 20 Kb

Multiple Temperature Activity

  • No

Thermal Cycler Type

  • Any

AffinityScript Multiple Temperature cDNA Synthesis Kit

The AffinityScript Multiple Temperature cDNA synthesis kit contains Agilent’s popular, highly thermostable, qPCR-grade AffinityScript Multiple Temperature Reverse Transcriptase for robust yields, from 37 to 60 °C, with nearly full activity between 45 to 55 °C. With the AffinityScript multiple temperature cDNA synthesis kit, Agilent offers enzymes with a broad thermal profile.

Features

  • Robust cDNA synthesis across a broad range of temperatures

  • Recommended up to 60 °C for RNA with increased 2 °C structure

  • Ideal for RT of GC-rich sequences

  • Consistently generates full length cDNA up to 20 kb

  • Sensitive detection of low-copy transcripts or limited RNA input

  • Strict purity specifications established to ensure no contaminating RNAse or exonuclease activities

  • This product can be customized to your needs.

Specialized kit

Discover Agilent’s range of qPCR specialty kits and reagents, like the MycoSensor qPCR assay kits, the miRNA qRT-PCR detection reagents, the porcine detection kit, and the qPCR NGS library quantification kit. All our real-time PCR specialty kits and reagents are fast, efficient, and deliver high specificity. The qPCR NGS library quantification kit can measure libraries at low concentrations (one femtomolar). Our MycoSensor qPCR assay kits detect Mycoplasma infection of cell cultures in fewer than two hours, while the miRNA qRT-PCR detection kit is a high-specificity reagent system for the detection of miRNA with fast results.

  1. Food and Agriculture Detection kit

  2. miRNA Detection kit

  3. qPCR NGS Quantitation kit

  4. Mycosensor qpcr Detection Kit

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+603-7610 0388

Unit C109-110, Level 1, Block C,

Kelana Square,

17, Jalan SS7/26, Kelana Jaya,

47301 Petaling Jaya, Selangor, Malaysia.

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