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Sequencer

DNBSEQ-T7.png

PRODUCT MODEL

DNBSEQ-T7

FEATURES

Ultra-High Throughput

OPERATION MODE

Ultra-High Throughput

MAX. THROUGHPUT/RUN

6TB

FLOW CELL TYPE

FC

EFFECTIVE READS/FC

5000M

AVERAGE RUN TIME

24-30 hours for PE150

MIN. READ LENGTH

PE100

MAX. READ LENGTH

PE150

DNBSEQ-T7.png

PRODUCT MODEL

DNBSEQ-G400

FEATURES

Adaptive

OPERATION MODE

High Throughput

MAX. THROUGHPUT/RUN

1440GB

FLOW CELL TYPE

FCL & FCS

EFFECTIVE READS/FC

1500-1800M

AVERAGE RUN TIME

FCS: 13-37 hours
FCL: 14-109 hours

MIN. READ LENGTH

SE50

MAX. READ LENGTH

SE400/PE200

DNBSEQ-T7.png

PRODUCT MODEL

DNBSEQ-G50

FEATURES

Effective

OPERATION MODE

Medium Throughput

MAX. THROUGHPUT/RUN

150GB

FLOW CELL TYPE

FCL & FCS

EFFECTIVE READS/FC

500M/ 100M

AVERAGE RUN TIME

9-40 hours

MIN. READ LENGTH

SE50

MAX. READ LENGTH

PE150

Introduction to MGI Sequencing Technology
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MGI's DNA sequencing instruments utilize the state-of-the-art core technology called DNBSEQ™. DNBs (DNA nanoballs) are pumped with by the fluidics system and loaded onto a Patterned Array chip. Sequencing primer is then added and hybridized to the adaptor region of the DNB. The sequencing reaction starts by pumping sequencing reagents containing fluorescently labeled dNTP probes and DNA polymerase.  Images are taken after the fluorescently labeled probes on the DNB are excited with lasers.  The images are then converted into a digital signal using MGI's propriety software. This information is then used to determine the DNA sequence of the sample.

All sequencing technologies relating to DNBs are part of DNBSEQ™. It includes: DNA single strand circularization and DNB preparation technology, Patterned Arrays, DNB loading, cPAS (combinatorial Probe Anchor Synthesis), Pair-End Sequencing technology on DNBs, fluidics and imaging systems, base calling algorithms, etc. cPAS technology has been widely used on various sequencing platforms including BGISEQ-50, BGISEQ-500, DNBSEQ-G50, DNBSEQ-G400, DNBSEQ-T7, etc.

Compared to other existing sequencing platforms, DNBSEQ™ sequencing technology combines the advantages from low amplification error rates from DNBs and high density patterned arrays. These advantages dramatically improve sequencing accuracy, and have much lower duplication rates in WGS/WES applications. When combined with the PCR-free library construction method, DNBSEQ™ also has higher SNP and indel detection accuracy compared to other platforms. In addition, the index hopping rate in DNBSEQ™ platforms is much lower as compared to that of other platforms.  

Sequencer
Product Model
Features
Operation Mode
Max. Throughput/run
Flow Cell Type
Effective Reads/ FC
Average run time
Min Read Length
Max Read Length
Ultra-High Throughput
Ultra-High Throughput
6TB
FC
5000M
24-30 hours for PE150
PE100
PE150
Adaptive
High Throughput
1440GB
FCL & FCS
1500-1800M
FCS: 13-37 hours FCL: 14-109 hours
SE50
SE400/PE200
Effective
Medium Throughput
150GB
FCL & FCS
500M/ 100M
9-40 hours
SE50
PE150