IMMUNOVERSE

IMMUNE

REPERTOIRE CHARACTERIZATION

unbiased amplification for a true and reproducible measure of diversity

SENSITIVE

CLONOTYPE IDENTIFICATION

AMP powered error correction provides a true measure of sample complexity and enables rare clone identification

COMPREHENSIVE COVERAGE

Archer molecular bar coded adapter contain universal primer binding sites allowing for open - ended amplification and sequencing throughout entire V(D)J region, resulting in increase coverage of CDR3

FLEXIBLE INPUT

Input amounts range 25ng to 2ug of RNA from PBMC, FFPE or fresh frozen tissue sample types

MULTIPLE APPLICATIONS

Archer immunoverse can be used for disease tracking, repertoire profiling and immune reconstitution

Platform

Description

Illumina®

8-reaction panel

Immunoverse-HS TCR

Immunoverse-HS BCR

Immunoverse™ IGH

Immunoverse™ IGK/L

TCR Panel

Immunoverse TCR panels amplify T cell receptor beta/gamma or alpha/delta chains for sequencing and analysis across a wide variety of clonality and diversity applications. With targeted primers within 100 bp of the end of the CDR3 sequence of interest, the beta/gamma panel is designed for maximal sensitivity in FFPE material to profile tumor infiltrating lymphocytes (TILs). Sample inputs range from 25ng to 2μg.

BCR Panel

Immunoverse BCR panels amplify B cell receptor heavy (IGH) and light (kappa/lambda) chains for sequencing and analysis. Primers are designed to identify isotypes and subclasses in the Fc region and somatic hypermutations within the variable regions. Sample inputs range from 25ng to 6μg.