RNA

Whole Transcriptome

RNA-Seq Library Preparation Kits

SureSelect XT HS2 mRNA Library Preparation Kit

The SureSelect XT HS2 mRNA Library Preparation Kit is Agilent’s newest strand-specific, poly-A enriched mRNA-Seq solution for Illumina sequencing. It targets the coding regions in the transcriptome for gene expression profiling, fusion detection and novel transcript discovery.

The kit offers a streamlined workflow that generates high quality libraries with excellent library complexity and uniform transcript coverage using 10 – 1000ng of total RNA. 384 unique dual indexes allow you to minimize index hopping. It is equipped with molecular barcodes to identify PCR artifacts.

Features

  • Generate strand-specific mRNA libraries with 10 - 1000 ng of total RNA.

  • Streamline your workflow with a simple protocol.

  • Improve sequencing performance with high library complexity.

  • Minimize index hopping with 384 unique dual indexes.

  • Identify PCR artifacts with molecular barcode for accurate gene expression.

Specifications

Automation Compatible

  • Yes

Includes Molecular Barcode

  • Yes

Multiplexity

  • 384 Unique dual indexes

Sample Input

  • 10-1000 ng

Strand Specificity

  • Yes

Tissue Sample Type

  • Fresh Frozen Tissue

  • Blood

Turnaround Time

  • 5.5 hr

 

NGS Custom Target Enrichment Probes

SureSelect Custom RNA Target Enrichment Probes

SureSelect Custom RNA Target Enrichment Probes enrich your cDNA library for specific low-abundance transcripts and gene fusions of interest. This targeted RNA sequencing approach improves sequencing coverage, read depth, and enhances sensitivity for what matters to you while minimizing sequencing cost.

Our expert design team can help you design and order Custom RNA Target Enrichment Probes specifically designed with your experiment in mind. Your design can then be used to enrich cDNA libraries generated with the SureSelectXT RNA Direct Library Preparation kit.

Features

  • Create custom RNA target enrichment probes to enrich for transcripts and gene fusions important to you

  • Improve RNA sequencing coverage, read depth, and enhance sensitivity

  • Agilent’s expert design team can help you create a custom panel to best suit your needs and lead you through the ordering process

Specifications

Number of Enrichments

  • 16 - 480

Number of Hybridizations

  • 16 - 480

Species

  • Eukaryotes

  • Prokaryotes

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