RNA

Whole Transcriptome

RNA-Seq Library Preparation Kits

SureSelect XT HS2 mRNA Library Preparation Kit

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The SureSelect XT HS2 mRNA Library Preparation Kit is Agilent’s newest strand-specific, poly-A enriched mRNA-Seq solution for Illumina sequencing. It targets the coding regions in the transcriptome for gene expression profiling, fusion detection and novel transcript discovery.

The kit offers a streamlined workflow that generates high quality libraries with excellent library complexity and uniform transcript coverage using 10 – 1000ng of total RNA. 384 unique dual indexes allow you to minimize index hopping. It is equipped with molecular barcodes to identify PCR artifacts.

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Features

• Generate strand-specific mRNA libraries with 10 - 1000 ng of total RNA.

• Streamline your workflow with a simple protocol.

• Improve sequencing performance with high library complexity.

• Minimize index hopping with 384 unique dual indexes.

• Identify PCR artifacts with molecular barcode for accurate gene expression.

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Specifications

Automation Compatible

• Yes

Includes Molecular Barcode

• Yes

Multiplexity

• 384 Unique dual indexes

Sample Input

• 10-1000 ng

Strand Specificity

• Yes

Tissue Sample Type

• Fresh Frozen Tissue

• Blood

Turnaround Time

• 5.5 hr