Reagents for PCR

PCR-grade dNTP and 10x DNA Polymerase Buffers

dNTP mixes

Agilent PCR-grade dNTP and 10x DNA Polymerase Buffers include 25 mM of each standard dNTP (GATC) for use with any PCR application, including real-time quantitative PCR. These reaction buffers contain the optimal magnesium concentration for efficient PCR.

Features

  • Optimizes PCR performance

  • Produces high-quality reaction products

  • Withstands multiple freeze-thaw cycles without compromising efficiency

 

PCR-grade dNTP and 10x DNA Polymerase Buffers

10X polymerase buffer

Agilent PCR-grade dNTP and 10x DNA Polymerase Buffers include 25 mM of each standard dNTP (GATC) for use with any PCR application, including real-time quantitative PCR. These reaction buffers contain the optimal magnesium concentration for efficient PCR.

Features

  • Optimizes PCR performance

  • Produces high-quality reaction products

  • Withstands multiple freeze-thaw cycles without compromising efficiency

PCR Enzymes and Reagents for Speed, Specificity, Yield, and Fidelity

RNA polymerases

1. RNAMaxx High Yield Transcription Kit

Rapidly and easily produce high yields of RNA from a DNA template.

2. SP6 RNA Polymerase

A DNA-dependent RNA polymerase with high sequence specificity for SP6 promoter sequences.

3. T3 & T7 Polymerases

DNA-dependent RNA polymerases with high sequence specificity for T3 or T7 promoters.

PCR Cloning

StrataClone PCR Cloning Kit

The Agilent StrataClone PCR cloning kit enables high-efficiency, 5-minute cloning of PCR products at room temperature. The kits use the DNA rejoining activity of DNA topoisomerase I and the DNA recombination activity of Cre recombinase to yield >95% insert positive clones. Available for both blunt-end and UA cloning, the kits can clone both long and short amplicons.

In addition, our StrataClone Ultra PCR Cloning System is also available. This kit includes the PfuUltra II Fusion Hotstart DNA Polymerase. The StrataClone Ultra system combines the highest accuracy in PCR amplification with easy, reliable, and affordably priced topoisomerase-based cloning.

Features

  • Clone PCR products at room temperature in just 5 minutes.

  • Incorporates the DNA rejoining activity of DNA topoisomerase I and the DNA recombination activity of Cre recombinase.

  • Available for both blunt-end and UA PCR cloning.

  • High efficiency yields >95% insert positive clones.

  • Clone both long and short amplicons (500 bp - 9.2 Kb) with the same kit.

  • Includes Kanamycin and Ampicillin Resistance Markers.

Specifications

Cloning Efficiency

  • >95% percent

Cloning Technologies

  • Cre recombinase technologies

  • DNA topoisomerase I

Reaction Time

  • 5 min

Reactions per Kit

  • 20

PCR Purification Kit

Strataprep PCR Purification Kit

The StrataPrep PCR purification kits provide a rapid method to simultaneously purify PCR products from PCR primers, unincorporated nucleotides, buffer components, and enzymes.

Features

  • Accommodates purification of PCR products, from 100 bp to long PCR amplicons

  • Highly purified DNA, ready for use

  • No hazardous organic extraction or ethanol precipitation

  • Ideal purification for high throughput sequencing and microarrays

DNA Kit for qPCR

StrataPrep Plasmid Miniprep Kit

StrataPrep Plasmid Miniprep Kits, a rapid, phenol-free method for purifying plasmid DNA from bacterial cultures. Bacteria is first pelleted, lysed, and neutralized. Precleared lysate is then mixed with a DNA binding solution and subjected to microspin. Once bound, the purified DNA is washed, eluted, and ready for use.


Each StrataPrep Plasmid Miniprep Kit is optimized for a specific application for high-efficiency purification. Purified DNA is application-ready in minutes, with no need for aliquoting messy slurries, extracting with phenol, or concentrating the DNA via alcohol precipitations.

Features

  • For high yields of plasmid DNA - up to 20 µg from 1.5 to 3.0 mL of culture

  • Superior purity - DNA transforms E. coli with high efficiency and yields quality sequences using automated or manual methods

  • Accommodates DNA from 2.9 Kb plasmids to 45 Kb cosmids

  • Purified plasmids transform as efficiently as cesium chloride-purified DNA

  • Rapid protocol uses a modified alkaline lysis method

Specialized kit

PCR Enzymes and Master Mixes for All Your PCR Needs and the Best Results.

A wide variety of PCR polymerases and reagents, PCR-based specialty kits and reagents, PCR inhibitors, and cloning kits to serve your PCR DNA amplification needs.

1. DNA Fish ID Kits

DNA fish ID kits let you identify species quickly, easily, and accurately using a DNA-based method.

 

2. MycoSensor Assay Kits

Detect Mycoplasma infection of cell cultures by PCR in less than two hours.

 

3. SureDirect Blood PCR Kit

Enables direct DNA amplification from liquid blood, dried blood on paper, serum, plasma, and more.

DNA FISH ID Kit

RFLP Decoder software

The Agilent DNA Fish ID solution uses advanced software to achieve more accurate results - quickly and easily. Key to the process is the Agilent 2100 Bioanalyzer and RFLP Decoder Software that performs RFLP pattern matching, eliminating the need for manual calling.

Features

  • Includes a database of numerous experimentally derived fish species profiles and a separate database of in-silico-derived profiles

  • Enables restriction fragment length polymorphism (RFLP) analysis

  • Generate fragment patterns that can be resolved and matched to the correct species using RFLP pattern matching softwares

PCR Plastics and Supplies

StrataCooler LP Benchtop Cooler

The Agilent StrataCooler LP Benchtop Cooler has a capacity of 32 x 1.5 mL tubes and weighs 1.2 kg. The StrataCooler provides protection for your enzymes in the freezer or on the benchtop. It maintains -15 °C for at least 2 hours on the benchtop.

The StrataCooler cryo preservation module is offered with the benchtop cooler, and increases survival rates (up to 90 %) by enabling controlled freezing (0.4 –0.6 °C/min) of cells to -70 °C. The module provides for 32 standard cryovials. It weighs 1.0 kg and is 12 cm wide, 21 cm deep, and 8 cm.

Features

  • Provides the most protection for your enzymes in the freezer or on the benchtop

  • Maintains -15 °C for at least 2 hours

  • Compatible with the StrataCooler Cryo Preservation Module, which provides 32 standard cryo vial support for -70 °C storage

Specifications

Controlled Freezing

  • No

Depth

  • 21 cm

Dimensions (WxDxH)

  • 12 x 21 x 8 cm

Gradient Temperature Value

  • -15 °C

Height

  • 8 cm

Weight

  • 1.2 kg

Width

  • 12 cm

StrataCooler

Cell Freezing and Cryo Preservation

StrataCooler Cryo Preservation Module provides the capacity for 32 standard cryovials compatible with the StrataCooler LP Benchtop Cooler. The module weighs 1.0 kg, with dimensions (WxDxH) of 12 cm x 21 cm x 8 cm. StrataCooler cryo preservation module enables controlled freezing to -70 °C (0.4 - 0.6 °C/min) of cells to increase survival rates (up to 90 %).

The StrataCooler LP Benchtop Cooler has a capacity of 32 x 1.5 mL tubes and weighs 1.2 kg. The StrataCooler LP Benchtop Cooler provides the protection for your enzymes in the freezer or on the benchtop. It maintains -15 °C for at least 2 hours at the benchtop.

Features

  • Enables controlled freezing (0.4 - 0.6 °C per minute) of mammalian and insect cells to significantly increase survival rates (up to 90 %).

  • Weighs 1.0 kg, with dimensions (WxDxH) of 12 cm x 21 cm x 8 cm

Microcentrifuge Tube Block for Ice

Microcentrifuge Tubes Block

Ensure your working area is organized and temperature reliable with the Agilent Tube Rack.

PCR (Polymerase Chain Reaction) Tube Blocks are available for 600 µL tubes (holding 40 x 600 µL tubes) and for 200 µL tubes or U-bottom plates.

Features

  • Robust PCR Tube block for organized and efficient working environments.

  • Available for 600 µL tubes in racks holding 40 x 600 µL tubes.

  • Also available for use with 200 µL tubes or U-bottom plates.

Specifications

Number Tubes Held

  • 600

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+603-7610 0388

Unit C109-110, Level 1, Block C,

Kelana Square,

17, Jalan SS7/26, Kelana Jaya,

47301 Petaling Jaya, Selangor, Malaysia.

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