Exome Probes

SureSelect Human All Exon

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The Agilent SureSelect Human All Exon V7 delivers unmatched coverage of targeted regions with minimal sequencing. Designed using the GRCh38/hg38 genome assembly, this exome design targets only coding regions from RefSeq, CCDS, GENCODE &USC Known Genes. All exon sequencing product features a novel bait design algorithm resulting in an end-to-end design size of only 48.2 MB. A novel bait design algorithm results in a sleek design with an end-to-end design size of only 48.2Mb.

The exome sequencing system provides excellent uniformity and allows more samples to be pooled per flow cell, for more cost-effective sequencing. The accurate targeting ensures that even hard-to-capture exons can be accessed. In addition, the system is compatible with all SureSelect Library prep solutions, making it one of the most versatile exome solutions for a wide range of sample types including low input material and FFPE samples, and workflow needs such as enzymatic or mechanical shearing.

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Features

• With just 5.3 GB of sequencing, the SureSelect Human All Exon V7 provides 20x coverage of >94% of targeted bases.

• Excellent overall uniformity and percent on-target enable variant calling with high confidence while reducing sequencing costs.

• Targets protein coding regions from RefSeq, GENCODE, CCDS and UCSC known genes, including hard-to-capture exons that are omitted from other exomes.

• Targets all pathogenic variants in the genes included in the ACMG guidelines for secondary findings.

• The raw data generated from human all exon sequencing can be readily analyzed using the Agilent Alissa Clinical Informatics Software, solving the data analysis bottleneck.

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Specifications

Number of Reactions        16 - 96

Target Region                   35.7 Mb