PCR

Equipment

SureCycler 8800 Thermal Cycler

The SureCycler 8800 Thermal Cycler provides users with a complete package of market-leading features and functionality. This PCR machine can run even the most complex thermal cycling techniques including time and temperature increments, touchdown PCR, and temperature gradients.

Features

  • 12 temperature gradient functionality without sacrificing accuracy or uniformity

  • One instrument that offers both fast cycling (6°C/sec ramp) and large volumes capacity (10-100µL)

  • Modular 96-well and 384-well interchangeable blocks

  • 7' LCD touch screen, with intuitive easy-to-use software

  • Remote access to start, stop, program, and monitor experiments from most web-enabled devices

Specifications

Dimensions (WxDxH)

  • 41 L x 28 W x 27 (38 open) H

Display Resolution

  • 800 x 480 pixels

Display Size

  • 7 in

Display Type

  • Full Touch LCD

Gradient Temperature Range

  • 30 - 90 °C

Has Gradient Functionality

  • Yes

Has Interchangeable Well Modules

  • Yes

Height

  • 27 cm

Number of USB Ports

  • 2

Pre-loaded Protocols

  • Yes

Protocol Storage Count

  • 10000protocols

Remote Monitoring

  • Yes

Standard Warranty Length

  • 2 yr

Temperature Accuracy

  • ±0.2 °C

Temperature Uniformity

  • ±0.4 °C

USB Format

  • 0.2

Weight

  • 12.4 kg

Width

  • 28 cm

 

Reagents

PCR Master Mixes and Polymerases

High fidelity/ GC Rich target DNA polymerases

PCR Enzymes and Reagents for Speed, Specificity, Yield, and Fidelity

Polymerase chain reaction (PCR) enzymes and reagents provide a wide variety of routine, high-fidelity, and Hotstart transcription enzymes for all your PCR needs. These products support features such as reverse transcriptase cloning, cDNA from an RNA template, and high-fidelity cloning.

  1. Herculase II Fusion DNA polymerases

  2. PfuUltraII Fusion High-Fidelity DNA polymerase

  3. Herculase Enhanced DNA polymerase

  4. PicoMaxx high Fidelity PCR system

  5. PfuTurbo CX Hotstart DNA Polymerase

DNA polymerases

SureStart Taq DNA Polymerase

SureStart Taq DNA polymerase is a hot start Taq DNA polymerase. SureStart Taq can be incorporated into PCR protocols previously optimized with Taq DNA polymerase, with little or no modification of cycling parameters or reaction conditions.

Features

  • Eliminates amplification of background artifacts

  • Minimizes smearing in long PCR

  • Hot start increases specificity and sensitivity

  • Reliable room-temperature setup

DNA Polymerases for PCR

Paq5000 DNA Polymerase

Paq5000 is capable of quickly and robustly amplifying up to 6kb genomic DNA targets. With a shortened extension rate of 30 sec/kb and robust yields, Paq5000 is a Taq alternative that can be substituted easily into many Taq-based procedures.

Paq5000 includes the ArchaeMaxx factor, improving the yield of products by overcoming dUTP poisoning, which is caused by dUTP accumulation during PCR through dCTP deamination. Once incorporated, dU-containing DNA inhibits the enzyme and the ArchaeMaxx factor functions as a dUTPase, converting poisonous dUTP to harmless dUMP and inorganic pyrophosphate.

Features

  • Equal to better yield with fast cycling conditions

  • Robust, cost-effective alternative to Taq

  • Pre-optimized reaction buffers included

  • HotStart formulation

  • Mixed ends

DNA Polymerases for PCR​

Klenow, Full Size and Exonuclease Deficient

Choose the Klenow Fill-In Kit to perform both partial fill-in reactions to complete fill-in of a 5 overhang to generate blunt ends, and allow direct ligation. Or, use the kit to radioactively end label a 5 overhang with [alpha-32P]dNTP.

The Klenow Fill-in Kit contains Klenow fragment (125 U), dNTPs (separate 10 mM stocks), Klenow reaction buffer, predigested DNA, which can be used as a control, and sufficient material for 25 reactions.

Features

  • Perform a complete fill-in of a 5 overhang to generate blunt ends, to allow direct ligation

  • Radioactively end label a 5 overhang with [alpha-32P]dNTP

  • Includes predigested DNA that can be used as a control

  • The sample DNA should be extracted with phenol–chloroform and ethanol-precipitated before performing a fill-in reaction.

  • Add the Klenow polymerase to the reaction mixture last—after the DNA, fill-in buffer and dNTPs—and do not let the reaction mixture sit on ice for an extended period of time.

Specifications

Reactions per Kit

  • 25

Substrate Type

  • DNA

Unit Definition

  • Five units should be used with 1-1.5 µg of DNA

Reverse Transcriptases

AffinityScript One-Step RT-PCR Kit

The Agilent AffinityScript One-Step RT-PCR Kit provides a complete system for fast, high-yield, reliable single-tube RT-PCR. The system features a master mix format which, when combined with robust and rapid cycling conditions, makes it ideal for gene profiling and other high-throughput end-point RT-PCR applications.


Using the kit cDNA is synthesized from total or poly(A)+ RNA. This is carried out using a genetically engineered, highly thermostable version of MMLV RT called AffinityScript reverse transcriptase (RT). In addition, a special enzyme formulation and the kit's optimized buffer system ensure consistent and robust performance when amplifying difficult and GC-rich targets.

Features

  • Reduced PCR cycling times and a one-tube, master mix format that is designed to reduce contamination, increase throughput and save valuable research time.

  • High affinity for primer-template complexes, delivering optimal cDNA yield.

  • Combined performance of Herculase II fusion DNA polymerase and the ArchaeMaxx PCR enhancing factor, delivering superior PCR yield and reliability.

  • Robust performance for difficult and GC-rich targets.

  • Optimized for target lengths up to 9.5 KB.

  • The high fidelity of Herculase II fusion DNA polymerase results in low error rates for RT-PCR cloning applications.

Specifications

High Yield for GC-rich Sequences

  • Yes

Long-read

  • 20 Kb

Multiple Temperature Activity

  • No

Reactions per Kit

  • 100

Thermal Cycler Type

  • Any

Others

Ligases

PCR Enzymes and Reagents for Speed, Specificity, Yield, and Fidelity

Polymerase chain reaction (PCR) enzymes and reagents provide a wide variety of routine, high-fidelity, and Hotstart transcription enzymes for all your PCR needs. These products support features such as reverse transcriptase cloning, cDNA from an RNA template, and high-fidelity cloning.

1. Pfu DNA Ligase

High quality DNA ligase with higher ligation specificity and lower background than Tth DNA ligase.

2. T4 DNA Ligase

T4 DNA Ligase catalyzes 5’-phosphate and 3’-hydroxy bond formation of double stranded DNA and RNA

Nucleases

1. DNase I, RNase Free

DNase I, Rnase Free enzyme from bovine pancreas produces no noticeable RNA degradation.

2. RNace-IT Ribonuclease Cocktail

A mixture of two RNases: RNase A and a nonspecific RNase from Aspergillus oryzae known as RNase T1.

Nucleases Inhibitor

1. RNase Block

A 50-kDa protein that specifically inhibits common eukaryotic RNases.

Ladder

1 – 10 kb DNA Ladder

The DNA Ladder contains fragments from 2 to 10 kb at 1 kb increments and an additional higher band at 12 kb as well as bands at 250, 500, 750 and 1500 bp.
The higher bands are brighter than those at the lower end, with the transition to higher intensity at the 2 kb band serving as a useful reference point. Each fragment contains a 5' overhang (TTAA) that can be radioactively labeled using the Klenow Fill-In Kit. DNA fragments may also be detected via nonradioactive Southern blot analysis by incorporating fluor-modified nucleotides into the DNA prior to electrophoresis and using a tan Illuminator Chemiluminescent Detection System.

Features

  • Fragments are exact multiples or fractions of 1000 bp

  • Includes visual reference point for determining location

  • More economical than competitive markers

  • Easy radiolabeling of ladder

  • Known mass amount of each fragment for DNA quantitation

Specifications

Fragment Size

  • 1 - 10 Kb

Physical Form

  • Premixed

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+603-7610 0388

Unit C109-110, Level 1, Block C,

Kelana Square,

17, Jalan SS7/26, Kelana Jaya,

47301 Petaling Jaya, Selangor, Malaysia.

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